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Effect of Selected Dietary Buffers upon Utilization of Concentrate- or Roughage-Based Cattle Diets: Laboratory Studies^1,2,

Auburn University, Auburn, AL 36849

Abstract

Four chemical buffers were evaluated with in vitro rumen fermentation studies using both an 80% concentrate and a 50% roughage diet. Treatments included a positive control (PC), negative control (NC) and four buffered diets in which 500 mg of either CaHP0₄, CaC0₃, NaHC0₃ or Na₄P₂0₇ were added. The PC consisted of unbuffered diet with one part rumen fluid and four parts McDougall's artificial saliva. In the unbuffered NC and buffered treatments, three-fourths of the artificial saliva was replaced by isoosmotic saline. In the concentrate-based diet, NaHC0₃ and Na₄P₂O₇ elevated (P<.05) pH above the NC. Starch digestion and total VFA were increased (P<.05) by NaHC0₃ compared with the NC while the molar proportion of individual VFA was not altered. Tetrasodium pyrophosphate had no effect on starch digestion or total VFA, but did increase (P<.05) the molar proportion of acetic acid. Regarding the 50% roughage diet, both NaHC0₃ and Na₄P₂O₇ elevated (P<.05) starch and cellulose digestion and total VFA compared with the NC. Both NaHC0₃ and Na₄P₂O₇ increased (P<.05) the molar proportion of acetate to equal that of the PC. Tetrasodium pyrophosphate decreased (P<.05) apparent starch digestion compared with the NC, but increased (P<.05) the molar proportion of acetate. Compared with the NC, CaHP0₄ and Na₄P₂O₇ increased the quantity of microbial alpha-amino N in both diets. Soluble P was highly correlated with microbial protein synthesis in both the concentrate- and roughage-based diets (.92 and .90, respectively).

¹ Journal Paper No. 4-82269 of the Alabama Agr. Exp. Sta.

² Data taken in part from Matthew Hall's M.S. Thesis (1982). Research supported in part by the Monsanto Co., St. Louis, MO.

³ Current address is Federal Land Bank Assoc, Montgomery, AL 36116.

⁴ Dept. of Anim. and Dairy Sci.

⁵ The authors wish to acknowledge the technical assistance of Dr. John McGuire for statistical analysis and Mr. John Cunningham and Mr. Hershal Mooney and care of the animals.

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