J. Anim Sci.
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J. Anim Sci. 1984. 58:1432-1436.
© 1984 American Society of Animal Science
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Sex Ratio after Insemination of Bovine Spermatozoa Isolated using a Bovine Serum Albumin Gradient1

W. E. Beal2, Lydia M. White2,3, and Duane L. Garner4

Virginia Polytechnic Institute and State University, Blacksburg 24061

Abstract

This experiment was undertaken to determine if a method reported to successfully enrich the proportion of Y-chromosome-bearing spermatozoa in human semen could be adapted for separation of bovine spermatozoa. Semen was collected from four Angus bulls and aliquots were either separated on discontinuous gradients of bovine serum albumin (BSA) or untreated before processing for cryopreservation. Two hundred seventy -one cows or heifers were assigned randomly to be artificially inseminated (20 x 106 sperm/ insemination) with separated or unseparated spermatozoa. The proportions of male offspring were 45 and 54% after inseminations with separated or unseparated spermatozoa, respectively. In a second phase of the experiment, pooled semen from three Holstein bulls was either extended and frozen without separation or frozen after separation using the discontinuous BSA gradient. Separated and unseparated spermatozoa were analyzed by flow cytometry to determine the ratio of X- and Y-chromosome-bearing spermatozoa based on differences in DNA content. The ratios of X- and Y-bearing spermatozoa in separated or unseparated samples were indistinguishable. We concluded that the separation method did not enrich the proportion of Y-bearing bovine spermatozoa.

Footnotes

1 Appreciation is expressed to: Union Fork and Hoe Co. Foundation for financial support; Drs. B. L. Gledhill and D. Pinkel of the Livermore National Laboratory, Livermore, CA and Dr. L. A. Johnson, ARS-USDA, Beltsville, MD for assistance with the flow cytometry and to Mr. C. E. Marshall and Select Sires Inc., Plain City, OH for cooperation in collecting the semen.

2 Dept. of Anim. Sci.

3 Present address: Division of Anim. Sci., West Virginia Univ., Morgantown 26506.

4 Dept. of Physiol. Sci., Oklahoma State Univ., Stillwater 74074.






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Copyright © 1984 by the American Society of Animal Science.