J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1984. 58:714-724.
© 1984 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stern, M. D.
Right arrow Articles by Satter, L. D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Stern, M. D.
Right arrow Articles by Satter, L. D.

Evaluation of Nitrogen Solubility and the Dacron Bag Technique as Methods for Estimating Protein Degradation in the Rumen1

Marshall D. Stern2 and Larry D. Satter

University of Wisconsin3,4, Madison 53706

Abstract

Nitrogen solubility after 1 h extraction in 10% mineral buffer and N disappearance from Dacron bags placed in the rumen of a fistulated cow were evaluated as methods for predicting protein degradation in the rumen. In vivo measures of ruminal protein degradation in cattle and sheep fitted with duodenal cannulae were available for 34 diets, thus enabling direct comparisons between protein solubility, in situ methods and in vivo measurements of protein degradation. Dacron polyester bags used in this study had an average pore size of 52 µm and were removed from the rumen at intervals of 1, 4, 8, 12,17 and 24 h. Nitrogen disappearance at each interval of rumen exposure was correlated with N solubility. The correlation coefficient at 1 h of rumen exposure for all diets was .79 and decreased as exposure time increased. Correlations between N disappearance at 1, 4, 8, 12, 17 and 24 h of rumen exposure and in vivo measurements of ruminal protein degradation were –.08, .16, .29, .56, .55 and .68, respectively. The correlation coefficient between N solubility and in vivo protein degradation was .26. Several methods for using the Dacron bag technique to predict protein degradation were also investigated.

Footnotes

1 Research supported by the College of Agr. and Life Sci., Univ. of Wisconsin, Madison, and by the National Feed Ingredients Association, Des Moines, Iowa.

2 Present address: Dept. of Anim. Sci., Univ. of Minnesota, St. Paul 55108.

3 The authors express appreciation to Beth A. Jones for conducting nitrogen analyses and to Lesley Rode and Elana S. Stern for preparation of the dacron polyester bags.

4 Dept. of Dairy Sci.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Society of Animal Science.