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Evaluation of Two Seminal Collection Regimens for Mature Holstein Bulls¹

American Breeders Service Division, W. R. Grace & Co., DeForest, WI 53532

Abstract

Twenty mature Holstein bulls (3 to 10 yr old) were used to test the effect of two semen collection regimens on spermatozoal output, post-thaw percentage spermatozoal motility, and time needed to make the collections/week. For both regimens, six ejaculates/wk were collected using either three ejaculates/d, 2 d/wk, or two ejaculates/d, 3 d/wk. A three-period switchback experimental design was used. Each collection period for which measurements were taken was 3 wk and was preceded by a 2 wk period of acclimation. The total number of spermatozoa harvested per week was not significantly different (P>.05): 33.2 X 10⁹ when the bulls were collected two ejaculates 3 d/wk, compared with 33.9 X 109 three ejaculates 2 d/wk. Post-thaw progressive spermatozoal motility was 50.3 and 52.1% (P>.05), respectively. The average time per week to collect each bull was 73.6 and 83.7 min (P<.05), respectively.

¹ The authors wish to acknowledge P. Press and G. Causer of the Research Division, W. R. Grace & Co., for assistance with the statistical analyses, and the Research and Production staff of American Breeders Service for their technical assistance.