J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1983. 57:1006-1012.
© 1983 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bondioli, K. R.
Right arrow Articles by Wright, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bondioli, K. R.
Right arrow Articles by Wright, R. W., Jr.

In Vitro Fertilization of Ovulated and Ovarian Ovine Oocytes1

Kenneth R. Bondioli2 and Raymond W. Wright, Jr.

Washington State University3, Pullman 99164

Abstract

In vitro fertilization of ovine ovulated and ovarian oocytes was attempted after capacitation of ejaculated spermatozoa by a variety of methods. All sperm incubations and oocyte cultures were performed in a modified Krebs Ringer bicarbonate (KRB) medium. High ionic strength (HIS) medium was prepared by adding NaCl to provide an osmolarity of ç370 mOsmol/kg. All oocytes were obtained from progestogen-synchronized and follicle-stimulating hormone-treated ewes. Ovulated oocytes were recovered 64 h after progestogen withdrawal. Ovarian oocytes were recovered 45 h after progestogen withdrawal. Oocytes were incubated with spermatozoa in microdrops for 6 h, transferred to fresh medium and cultured for 48 h. In the first series of experiments, capacitation was attempted by either preincubation in KRB, washing spermatozoa with KRB medium or treatment of spermatozoa with HIS medium. Spermatozoa washed with KRB penetrated three of 61 (5%) oocytes and spermatozoa incubated in HIS medium penetrated eight of 209 (4%) oocytes. In the second series of experiments, spermatozoa were capacitated by incubation with ovine cumulus cells or in the uterus of a rabbit. A 3 3% fertilization rate was observed with spermatozoa recovered from the rabbit uterus and a 28% rate with spermatozoa incubated with cumulus cells. Treatment with HIS medium previously shown to capacitate bovine and rabbit spermatozoa failed to capacitate ram spermatozoa; however, fertilization was observed after capacitation by cumulus cells or in the rabbit uterus.

Footnotes

1 Scientific paper no. 6306. College of Agr. Res. Center, Washington State Univ., Pullman. Project 0313.

2 Present address: Granada Genetics Inc., Route 1, Box 99A, Marquez, TX 77865.

3 Dept. of Anim. Sci.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Society of Animal Science.