HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Magness, R. R. Articles by Ford, S. P. Search for Related Content PubMed PubMed Citation Articles by Magness, R. R. Articles by Ford, S. P.

Estrone, Estradiol-17β and Progesterone Concentrations in Uterine Lymph and Systemic Blood throughout the Porcine Estrous Cycle^1,2,

Iowa State University, Ames 50011

Abstract

This study was conducted to determine the concentrations of estrone (E₁, pg/ml), estradiol-17β (E₂β, pg/ml) and progesterone (P₄, ng/ml) in uterine lymph (UL) and systemic blood (SB) throughout the estrous cycle of gilts. Eighteen gilts were assigned randomly to surgery on d 0 or 1 (n = 5), 5 (n = 4), 11 (n = 5) or 15 (n = 4) postestrus (first day of estrus = d 0). At surgery a catheter was inserted into a uterine lymphatic closely associated with a main branch of the middle uterine artery supplying one uterine horn, above the anastomosis of the ovarian and uterine lymphatic systems. Lymph was allowed to flow continuously into a heparinized collection tube, which was replaced at 12-h intervals (1200 and 2400 h) until the loss of catheter patency ( 5 d). The two lymph samples collected daily from each gilt were pooled, and this pool was considered as the observation for that animal for that day. Systemic blood was collected via vena cava puncture at 1200 h on each day that lymph was obtained. Lymph and blood plasma were stored at -20 C until assayed for E₁, E₂β and P₄. Concentrations of E₁ E₂β and P₄ in UL were correlated (P<.01) with concentrations of the same steroid in SB throughout the estrous cycle (r = .61, .84 and .90, respectively). However, E₁ and E₂β were higher (P<.01) and P₄ was lower (P<.01) in UL than in SB. The ratio of estrogen (E₁ + E₂β to P₄ on a pg to ng basis, designated E:P₄, was greater during the follicular phase (5 d before estrus to 3 d postestrus) than during the luteal phase (d 4 to d 15 postestrus) of the estrous cycle in UL (19 vs 3.3) and SB (4 vs .3). These data demonstrate that concentrations of E₁, E₂β and P₄ in UL are highly correlated with concentrations of these ovarian steroids in SB throughout the porcine estrous cycle; however, the E:P₄ ratio is markedly higher in UL than in SB.

¹ This research fulfills partial requirements for the Ph.D. degree of R.R.M. It was presented in part at the 73rd Annu. Meet, of the Amer. Soc. of Anim. Sci. (1981), Raleigh, NC (Abstr. 5 35). Journal paper No. J-10717 of the Iowa Agr. and Home Econ. Exp. Sta., Ames; Projects 2443 and 2444.

² Appreciation is expressed to Dr. D. L. Hard and Mr. L. P. Reynolds for their assistance with sample collection and to Mr. W. G. McDonald for his technical guidance in the laboratory and to Denise Braymen for typing the manuscript.

³ Dept. of Anim. Sci.

This article has been cited by other articles:

				C. R. Rosenfeld, T. Roy, K. DeSpain, and B. E. Cox Large-Conductance Ca2+-Dependent K+ Channels Regulate Basal Uteroplacental Blood Flow in Ovine Pregnancy Reproductive Sciences, September 1, 2005; 12(6): 402 - 408. [Abstract] [PDF]

				R. R. Magness, T. M. Phernetton, T. C. Gibson, and D.-b. Chen Uterine blood flow responses to ICI 182 780 in ovariectomized oestradiol-17{beta}-treated, intact follicular and pregnant sheep J. Physiol., May 15, 2005; 565(1): 71 - 83. [Abstract] [Full Text] [PDF]