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Application of Radioimmunoassay (RIA) for the Determination of Residues of Anabolic Sex Hormones¹

Institut für Veterinärmedizin des Bundesgesundheitsamtes (Robert von Ostertag-Institut), D 1000 Berlin 33

Abstract

Based on the basic principles of radioimmunoassay (RIA), it can be expected that by using this method, residues of anabolic sex hormones in animal tissues can be quantitated in the ng/g to pg/g range with adequate reliability. This was demonstrated for various endogenous and exogenous steroids as well as for stilbene-estrogens. Using the RIA for DES as a regulatory method allowed the successful control of the misuse of this compound in animal production, especially by examination of excreta. Also, for muscular tissue, an interlaboratory validation study yielded acceptable results. The need for confirmatory methods is stressed.

¹ Paper presented at the Symposium on "Metabolic Fate of Anabolic Substances in Food Animals" at the meeting of the Aer. Soc. of Anim. Sci., Raleigh, NC, USA, on July 29, 1981.