J. Anim Sci.
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J. Anim Sci. 1983. 56:1261-1268.
© 1983 American Society of Animal Science
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Influence of Cooling Rates and Extenders upon Post-Thaw Viability of Bovine Spermatozoa Packaged in .25- and.5-ML French Straws1,2,

P. L. Senger, Jere R. Mitchell and J. O. Almquist

The Pennsylvania State University3, University Park 16802

Abstract

Post-thaw survival of bovine spermatozoa was compared for semen packaged in either .25-or .5-ml French straws and frozen at three different cooling rates in moving N2 vapor. Using a split ejaculate technique, nine ejaculates were extended in heated skim milk, egg yolk-citrate and egg yolk-Tris and packaged in .25- and .5-ml French straws. Semen packaged in the .25- and .5-ml straws was frozen simultaneously at initial N2 vapor temperatures of -140, -110 or -80 C. Semen was thawed in a water bath at 3 5 C for 1 min. Recovery of spermatozoa was evaluated immediately post-thaw (0 h) and again after 3 h of incubation at 37 C. Motility estimates and motility counts were made using phase contrast microscopy; percentage of spermatozoa possessing intact acrosomes was quantitated using differential interference contrast microscopy. There was a packaging unit x cooling rate interaction (P<.05) for all three viability measures. However, there was no consistent trend with regard to cooling rate or packaging unit among the three extenders examined. Post-thaw viability for each characteristic varied (P<.01) among extenders, but not for cooling rate or packaging unit (P>.05). Spermatozoa extended and frozen in egg yolk-Tris had greater (P<.05) post-thaw viability than those extended in skim milk or egg yolk-citrate regrardless of cooling rate or volume of the seminal package.

Footnotes

1 Journal Series No. 6504, Pennsylvania Agr. Exp. Sta. Project 2642.

2 We thank Atlantic Breeders Cooperative, NEBA and Sire Power, Inc. for financial support and I.M.V. International Corp. for donating the straws. Appreciation is expressed to J. C. Hartle for collection of semen and bull management and M. J. Briscoe and J. L. Peters for assistance in freezing and evaluation of the semen. Special thanks is extended to Joseph C. Stewart, Electronic Design Service Laboratory, The Pennyslvania State Univ. for designing and fabricating the heater grid.

3 Dairy Breeding Research Center, Dept. of Dairy and Anim. Sci.






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Copyright © 1983 by the American Society of Animal Science.