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On the Negative Feedback Regulation of Gonadotropins in Castrate and Intact Cattle with Comparison of Two FSH Radioimmunoassays¹

Cornell University⁴, Ithaca, NY 14853

² To whom reprint requests should be addressed

Abstract

Two homologous radioimmunoassays for bovine follicle stimulating hormone (bFSH) were utilized in comparing the differential regulation of FSH and luteinizing hormone (LH) in response to ovariectomy or administration of gonadal steroids in cattle. There appeared to be significant LH cross-reactivity in one of the bFSH systems (bFSH-HS-2-17), but not in the other (bFSH-BP3). Concentrations of FSH in plasma measured by these two systems suggested both qualitative and quantitative differences. Following ovariectomy in heifers, LH concentrations in plasma were increased by 7.5 h, while FSH (measured in the bFSH-BP3 system) was not significantly elevated until 18 h. Administration of 200 µg of estradiol-17β to ovariectomized heifers inhibited levels of FSH in plasma but large doses of testosterone (100 mg), androstenedione (400 mg) and dihydro-testosterone (800 mg) had no effect. Similarly, LH was not affected by the androgens, while estradiol induced LH surges, leading to increased mean LH concentrations. In contrast to the results in heifers, LH concentrations in plasma from steers were inhibited by administration of androgens as well as by estradiol. In steers, FSH (bFSH-BP3) was marginally inhibited by estradiol and not at all by the androgens. Differences in the secretory patterns of FSH and LH also occurred in intact heifers during the estrous cycle. The 72-h period preceding estrus (follicular phase) was characterized by rapidly declining serum progesterone concentrations, followed by concurrent increases in both LH and estradiol. The circulating levels of bFSH (BP3) tended to decline during this interval. Overall, during the estrous cycle, progesterone levels were positively correlated with bFSH-BP3 (r=.37) and negatively correlated with LH (r = –.39). The gonadotropins were not significantly related (r = –.15). These relationships are consistent with the concept that LH controls the final stages of follicular development in cattle and that FSH may exert only a permissive effect.

¹ The authors express appreciation to F. Michel, Valerie Nicol and D. King for technical assistance and to Gail Donnen for typing the manuscript. Reagents for bovine FSH radioimmunoassays were provided by Dr. K. W. Cheng, Univ. of Manitoba, Winnipeg, Manitoba, Canada and by Dr. D. J. Bolt, U. S. Dept. of Agr., Beltsville, MD. The authors are also indebted to Dr. L. E. Reichert, Jr. for providing purified ovine LH and to Dr. G. D. Niswender for supplying antisera for ovine LH and estradiol.

³ Present address: Dept. of Anim. Sci., Univ. of California, Davis 95616.

⁴ Dept. of Anim. Sci.