J. Anim Sci.
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J. Anim Sci. 1982. 55:1132-1138.
© 1982 American Society of Animal Science
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Effects of Steroids on Bovine T-Lymphocyte Blastogenesis in Vitro1,2,

Finnie Murray3 and John R. Chenault4

Ohio Agricultural Research and Development Center, Wooster 44691,3 and and The Upjohn Company,4, Kalamazoo, MI 49001

Abstract

Day 13 to 24 bovine concetuses convert C-19 and C-21 neutral steroids to 5ß-reduced steroids with great efficiency. Pregnancy steroids have been reported to be immunomodulatory in several species. This study examined the possibility that 5ß-reduced products of bovine conceptus steroid metablism, precursors, related steroids, progesterone or cortisol might affect bovine T-cell blastogenesis. The steroids tested were 5{alpha}-androstan-17ß-ol-3-one, androstene-3, 17-dione, 5ß-androstan-3{alpha}-ol-17-one, cortisol, progesterone, 5ß-pregnane-3,20-dione and 5ß-pregnane-3{alpha},20{alpha}-diol. The ability of each steroid, over a dose range of 10-2 to 104 nM, to affect phytohemagglutinin (PHA)-induced or concanavalin A (Con A)-induced bovine T cell blastogenesis, or the mixed lymphocyte reaction (MLR) was evaluated. Five lactating Holstein cows served as lymphocyte donors for mitogen studies and two, that exhibited strong MLR, were donors forthe MLR evaluation. Of the seven steroids tested none affected blastogenesis in a dose-related manner except for cortisol, which suppressed Con A-induced lymphocyte transformation as well as the MLR. Cortisol did not affect PHA-induced blastogenesis. Thus, of the pregnancy steroids tested at the concentrations noted, none had significant immunomodulatory effects.

Footnotes

1 Approved for publication as Journal Article no. 186-81 of the Ohio Agr. Res. and Develop. Center, Wooster 44691.

2 The authors are grateful to K. J. Luce for technical assistance.

3 Dept. of Anim. Sci.

4 Agricultural Division, Performance Enhancement Research.






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Copyright © 1982 by the American Society of Animal Science.