J. Anim Sci.
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J. Anim Sci. 1982. 55:891-900.
© 1982 American Society of Animal Science
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Effect of Acetylcholine, Prostaglandin F2{alpha} and Estradiol on Number of Sperm in the Reproductive Tract of Inseminated Rabbits

H. W. Hawk, B. S. Cooper and H. H. Conley1,2,

US Department of Agriculture3, Beltsville, MD 20705

Abstract

Acetylcholine, injected im into does immediately after natural mating, significantly increased the number of sperm recovered 1 h later from the vagina and cervices but not from the oviducts. Acetylcholine caused immediate increases in the frequency and amplitude of uterine contractions as measured by strain-gauge force transducers attached to the serosa and recorded by physiograph; contraction patterns returned to the preinjection pattern within 30 min. Administration of prostaglandin F2{alpha} (.75 mg) at the time of insemination increased sperm numbers significantly at 2.5 h in the oviducts, uterus and cervices; .15 or 3.75 mg caused relatively little increase in sperm numbers. Prostaglandin F2{alpha} increased the number and strength of uterine contractions for more than 30 min. Estradiol injected 1 h before artificial insemination increased the number of sperm recovered from the oviducts, uterus, cervices and vagina at 2.5 h after insemination. Estradiol caused little if any detectable increase in the frequency or strength of uterine contractions during the period of increased retention and transport of sperm. When does were inseminated with low numbers of sperm (86,000), prostaglandin F2{alpha} and phenylephrine, used for comparison, each increased the ovum fertilization rate (22% for control does; 76 and 73%, respectively, for prostaglandin F2{alpha}- or phenylephrine-treated does).

Footnotes

1 Reprod. Lab., Anim. Sci. Institute, Beltsville Agr. Res. Center, ARS.

2 The authors wish to thank Douglas Caldwell for capable technical assistance and Linda Neuenhahn for typing the manuscript.

3 Mention of companies or products in this report does not constitute endorsement by the USDA to the exclusion of others not mentioned.






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Copyright © 1982 by the American Society of Animal Science.