J. Anim Sci.
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J. Anim Sci. 1982. 55:369-375.
© 1982 American Society of Animal Science
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Variation in Porcine Zona Pellucida Morphology Following Pronase Treatment1,2,

A. R. Menino, Jr.3 and R. W. Wright, Jr.

Washington State University4, Pullman 99164

Abstract

The effects of cell stage and day of embryo collection on the pronase-induced zona pellucida morphology in pig embryos were investigated. Three hundred and seventy-two two-to eight-cell embryos were treated for 3.0 min in 5.0% pronase in Whitten's Medium. Responses in zona pellucida morphology observed after pronase treatment were: (1) absent, (2) stretched and (3) intact. Each pig was coded as to the type of zona pellucida morphology its embryos possessed after enzyme treatment according to the following scale: (1) — only intact, (2) — intact and stretched, (3) — only stretched, (4) — stretched and absent and (5) — only absent. Also, the number of embryos of a particular cell stage was expressed as a fraction of the total number of embryos collected from each pig. The incidence of intact zonae pellucidae was greatest (P<.05) among two- to three-cell embryos and the incidence of absent zonae pellucidae was greatest (P<.05) among six-to eight-cell embryos. A significant correlation (r = .79) was observed between day of embryo collection and coded zona pellucida morphology. The results suggest that the pronase-induced zona pellucida morphology observed is dependent on cell stage and day of embryo collection.

Footnotes

1 Scientific Paper No. 5998. College of Agr. Res. Center, Washington State Univ., Pullman. Project No. 0313.

2 The authors wish to thank the Washington State Pork Producers for contributions to this study.

3 Present address: College of Agr., Univ. of Hawaii at Hilo, Hilo 96720.

4 Dept. of Anim. Sci.






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Copyright © 1982 by the American Society of Animal Science.