J. Anim Sci.
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J. Anim Sci. 1982. 55:232-235.
© 1982 American Society of Animal Science
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Effect on Fertility of Freezing Large Numbers of Sraws of Bovine Spermatozoa in a Mechanical Freezer1

J. O. Almquist, C. H. Allen2 and R. J. Branas3,4,

The Pennsylvania State University5, University Park 16802

Abstract

Fertility of semen frozen in a mechanical forced-vapor freezer was compared with that frozen in static nitrogen (N) vapor. Semen from 10 Holstein bulls was extended in milk-10% glycerol at 30 x 106 progressively motile spermatozoa/ml and packaged in .5-ml French straws. Straws were divided into three equal groups • bull-1 • collection day-1 and frozen in a mechanical freezer at full or half loads (7,250 ± 250 or 3,500 ± 250 straws in 5-straw goblets on metal canes) or in static N vapor (330 ± 30 straws held singly on horizontal racks). For field distribution, straws frozen in static N vapor also were placed in 5-straw goblets on canes after being frozen. Each of the two goblets on a cane held a different treatment. Based on 75-d nonreturn rates for 23,137 first service inseminations, fertility was 1.6 percentage points higher (P<.05) for semen frozen in static vapor (66.3%) than that frozen in a fully loaded mechanical freezer (64.7%). The difference of 1.3 percentage points in fertility favoring static vapor over freezing a half load in a mechanical freezer (65.0%) approached significance (P = .06); half and full loads did not vary (P>.05). In conclusion, the small increase in fertility favoring static over forced-vapor freezing supports use of the static vapor method unless the savings in time and labor of freezing large numbers of straws at one time in a mechanical freezer are considered to offset the small difference in fertility.

Footnotes

1 Pennsylvania Agr. Exp. Sta., Journal Series No. 6323.

2 Atlantic Breeders Cooperative, 1575 Apollo Drive, Lancaster, PA 17601.

3 816 East South St., York, PA 17403.

4 Appreciation is expressed to David Yoder, his staff and the technicians of Atlantic Breeders Cooperative, Lancaster, PA for their assistance and cooperation and to J. C. Aubuchon for the statistical analysis.

5 Dairy Breeding Research Center, Dept. of Dairy and Anim. Sci.






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Copyright © 1982 by the American Society of Animal Science.