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Kansas State University, Manhattan 66506
Abstract
A method is described that provides a rapid and accurate determination of 2-aminoethylphosphonic acid (AEP) in feeds and in rumen bacteria and protozoa. A wide variety of feeds examined was devoid of AEP. Six rumen-fistulated cattle were fed either a high roughage (85% hay) or a high concentrate (85% concentrate) diet. Protozoa from cattle fed the high concentrate diet contained more (P<.05) AEP-N in relation to total N (.16%) than did those fed the high roughage diet (.10%). Protozoa from cattle fed the high concentrate diet contained less total N (7.8 vs 8.4%, P<.16) and more lipid (6.2 vs 3.0%, P<.05) than protozoa from cattle fed the high roughage diet. Amino acid N as a percentage of total protozoal N was greater (P<.05) for cattle fed the high roughage diet than for those fed the high concentrate (65.6 vs 57.9%). AEP was .33% of the amino acids in protozoa from cattle fed high concentrate, compared with .19% in protozoa from catde fed high roughage (P<.05). We concluded that to obtain accurate predictions of protozoal production in the rumen based on AEP, it will be necessary to correct for dietary effects on chemical composition of protozoa.
1 Contribution 81-418-j, Ruminant Nutr. Group, Dept. of Anim. Sci. and Ind., and Dept. of Statist., Kansas Agr. Exp. Sta.
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