J. Anim Sci.
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J. Anim Sci. 1982. 54:837-840.
© 1982 American Society of Animal Science

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Content of 2-Aminoethylphosphonic Acid in Feeds, Bacteria and Protozoa and its Role as a Rumen Protozoal Marker1

G. S. Dufva, E. E. Bartley, M. J. Arambel, S. J. Galitzer and A. D. Dayton

Kansas State University, Manhattan 66506

Abstract

A method is described that provides a rapid and accurate determination of 2-aminoethylphosphonic acid (AEP) in feeds and in rumen bacteria and protozoa. A wide variety of feeds examined was devoid of AEP. Six rumen-fistulated cattle were fed either a high roughage (85% hay) or a high concentrate (85% concentrate) diet. Protozoa from cattle fed the high concentrate diet contained more (P<.05) AEP-N in relation to total N (.16%) than did those fed the high roughage diet (.10%). Protozoa from cattle fed the high concentrate diet contained less total N (7.8 vs 8.4%, P<.16) and more lipid (6.2 vs 3.0%, P<.05) than protozoa from cattle fed the high roughage diet. Amino acid N as a percentage of total protozoal N was greater (P<.05) for cattle fed the high roughage diet than for those fed the high concentrate (65.6 vs 57.9%). AEP was .33% of the amino acids in protozoa from cattle fed high concentrate, compared with .19% in protozoa from catde fed high roughage (P<.05). We concluded that to obtain accurate predictions of protozoal production in the rumen based on AEP, it will be necessary to correct for dietary effects on chemical composition of protozoa.


Footnotes

1 Contribution 81-418-j, Ruminant Nutr. Group, Dept. of Anim. Sci. and Ind., and Dept. of Statist., Kansas Agr. Exp. Sta.







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Copyright © 1982 by the American Society of Animal Science.