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University of Florida, Gainesville 32611
Abstract
Development of the ovine conceptus was confined to the uterine horn ipsilateral to the corpus luteum (CL) by placing a ligature around that uterine horn at a point near the uterine body on day 5 of pregnancy. On day 140 of gestation, seven of 10 ewes were still pregnant and from 21 to 815 ml of uterine fluid (488 ± 94 ml, 
± SEM) were collected from the nongravid uterine horn. Total recoverable protein ( ± SEM) was 13.4 ± 3.4 grams. Polyacrylamide gel electrophoresis of the reduced proteins in presence of sodium dodecyl sulfate indicated that protein composition of uterine fluid was distinct from that of colostrum, serum, amniotic fluid, and allantoic fluid, and revealed the presence of two major polypeptides with molecular weights of about 57,000 and 58,500, respectively, plus numerous other minor components. Gel filtration on columns of Sephadex G-200 and Sepharose CL-6B suggested that these polypeptides formed a series of aggregates of high molecular weight when kept under nonreducing conditions. Glucose (.18 ± .03 mg/ml), but not fructose, was present in uterine fluid. In addition, high levels of prostaglandin F (451.4 ± 83.3 ng/ml) were present.
1 This work was supported in part by National Institute of Health Grants HD08560 (F.W.B. and R.M.R.) and HD05659 (D.C. and D.H.B.) and a grant from the foundation of Child Development (D.H.B.). D. Caton is recipient of Career Development Award K05-HD-70552-04. Florida Agr. Exp. Sta. Journal Series No. 1535.
The authors thank Ms. Lillian Dennison for typing this manuscript and Mr. Warren C. Clark for technical assistance.
3 Dept. of Biochemistry and Molecular Biology.
4 Dept. of Obstetrics and Gynecology.
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