J. Anim Sci.
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J. Anim Sci. 1979. 49:1021-1029.
© 1979 American Society of Animal Science
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Secretion of Insulin by the Nonruminant Herbivore (Pony) Pancreas Perfused in Vitro

Daniel C. Holley1 and J. Warren Evans2,3,

University of California, Davis 95616

Abstract

A technique is described to rapidly remove the pancreas from fed, adult Shetland ponies and to perfuse it in vitro. Thirty minute square-wave glucose stimuli (300 or 800 mg/dl) resulted in a dose dependent pattern of insulin secretion. The initial spike was similar to that observed in other mammals. Subsequent secretion, however, gradually decreased in relation to the initial spike. Butyrate (253 µM/liter) stimulated insulin secretion in the absence of glucose, whereas acetate (19.2 mM/liter) or propionate (505 µM/liter) failed to stimulate insulin secretion. The pony (a nonruminant herbivore) derives much of its energy from VFAs produced by microbial fermentation in the cecum and large colon. They have a digestive system with factors common to both ruminant and monogastric species. The perfused pony pancreas demonstrates insulin control mechanisms with aspects similar to both of these groups.

Footnotes

1 Present address: Department of Biological Sciences, San Jose State Univ., San Jose, CA 95192.

2 Department of Animal Science, Univ. of California, Davis 95616.

3 We are grateful to Dr. V. E. Mendel for his advice and many helpful suggestions. Thankful appreciation goes to Mr. Bill Parker whose surgical expertise proved indispensible. Special acknowledgement is due to Dr. D. L. Curry for many hours of stimulating discussion through all phases of this project.






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Copyright © 1979 by the American Society of Animal Science.