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Effect of Amino Acid Pattern and Level in Intravenously and Intraruminally Infused Sheep^{1 ,2,}

University of Missouri^,5, Columbia 65211

⁴ Person to whom reprint requests should be addressed.

Abstract

Six sheep were used in a 2 x 3 factorial experiment arranged in a 6 x 6 Latin square with an extra period. Two intravenous amino acid patterns (plasma vs tissue) and three levels of amino acid infusion (25, 50 or 75 g/day) were evaluated with respect to their effect on whole blood free amino acids and nitrogen metabolism. The sheep, having their rumens rendered nonfermentive, were dually infused: glucose, amino acids and water-soluble vitamins were administered intrajugularly ; volatile fatty acids, buffer and minerals were infused intra-ruminally. Each treatment combination of amino acid pattern and level was given for 3 consecutive days. Data were determined on the second and third days of each infusion period.

Nitrogen balance was greater (P<.05) when the tissue pattern was infused compared to the plasma pattern (2.47 vs 1.21 g/day). Infusion of the tissue pattern lowered (P<.05) urinary nitrogen (4.27 vs 6.03 g/day), urinary area nitrogen (3.40 vs 4.52 g/day) and plasma urea nitrogen (4.38 vs 7.17 g/100 ml). Generally, as the level of infusion was increased, corresponding increases were also seen in plasma urea nitrogen (4.17, 5.98, 7.18 g/100 ml), urinary nitrogen (4.18, 5.09, 6.17 g/day), nitrogen balance (–.68, 1.90, 4.31 g/day) and urinary urea nitrogen (2.97, 4.06, 4.86 g/day).

The tissue pattern more nearly represented the animal's optimum pattern than did the plasma pattern and increasing the infusion level from 25 to 50 or 75 g/day led to improved nitrogen balance.

¹ Contribution from the Missouri Agr. Exp. Sta. Journal Series No. 8127. Approved by the director. Supported in part by USPHS, NIH Grant 5.R01 AMI 6560-02.

² A portion of these data was presented at the 61st Annu. Meet. of the Federation of American Societies for Experimental Biology, April 1–8, 1977. An abstract was published in Fed. Proc. 36:1154.

³ Present address: Animal Science Research, International Minerals and Chemical Corp., Terre Haute, IN 47808.

⁵ Department of Animal Husbandry, Animal Science Research Center.