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Myofibrillar Protein Synthesis and Assembly in Satellite Cell Cultures Isolated from Skeletal Muscle of Mice¹

Michigan State University, East Lansing 48824

Abstract

Several developmental properties of replicating myogenic cells isolated from the hind limb muscles of neonatal, rapidly growing and young adult mice were examined in cell culture. Satellite cell progeny fused to form multinucleated myotubes that elaborated myofibrillar proteins and underwent spontaneous contractile activity. Additionally, the maximum extent of fusion was similar in cultures isolated from the three ages of mice when cultures were treated with fluorodeoxyuridine (FdU) to inhibit replication of non-muscle cells. However, the onset of fusion was delayed in the rapidly-growing and adult cultures compared to neonatal cultures. This differential onset of fusion was attributed to a higher proportion of non-muscle cells in the cell preparations from the older mice rather than to inherent cellular differences in fusion capability. To determine if the rates of myofibrillar protein synthesis and total protein synthesis were influenced by the age of mice from which satellite cells were isolated, cultures were pulse labeled with [³H] leucine. The distribution of polypeptides synthesized by these cells, the rate of myosin heavy chain synthesis and the rate of total protein synthesis were not appreciably affected by the age of mouse from which cells were isolated. Thus, it is concluded that the intrinsic myofibrillar protein synthetic capacity of satellite cell progeny is unaffected by mouse age.

¹ Michigan Agricultural Experiment Station Journal Article No. 8617. This research was supported in part by funds from the Muscular Dystrophy Association of America, Michigan Agricultural Experiment Station Project No. 1241 and 1265, and Biomedical Research Support Grants from the College of Agriculture and Natural Resources and the College of Osteopathic Medicine. Assistance with some of the experiments by Teresa Phillips, Anne Tuomari and Dianne Young is gratefully appreciated.

² Department of Animal Husbandry.

³ Department of Biomechanics.

⁴ Department of Food Science and Human Nutrition.

⁵ To whom all correspondence should be addressed: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, 48824.