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Myoglobin Heterogeneity in Pigs with PSE and Normal Muscle by an Improved Isoelectric Focusing Technique¹

Utah State University, Logan 84322^,2 and ; University of Minnesota, St, Paul 55108^,3

Abstract

Cardiac myoglobin heterogeneity was studied on purebred Pietrain and Minnesota No. 1 pigs, and seven cross-bred groups of these breeds. Two isoelectric focusing procedures were used, one with urea and one without. Both procedures employed polyacrylamide and a heme-specific stain (o-diansidine). Satisfactory results were obtained without urea. A heterogeneous banding pattern consisting of eight myoglobin bands was obtained with 700 µg of protein from heart extracts (.001 M KCN), with similar patterns observed for the 62 pigs among all breed groups. Further studies revealed that banding patterns were similar for cardiac and skeletal muscle and between pale, soft, exudative (PSE) and normal muscle at 5 min and 24 hr postmortem. The results suggest that previously reported variants in myoglobin in PSE skeletal muscle are not the result of alterations in primary structure.Cardiac myoglobin heterogeneity was studied on purebred Pietrain and Minnesota No. 1 pigs, and seven cross-bred groups of these breeds. Two isoelectric focusing procedures were used, one with urea and one without. Both procedures employed polyacrylamide and a heme-specific stain (o-diansidine). Satisfactory results were obtained without urea. A heterogeneous banding pattern consisting of eight myoglobin bands was obtained with 700 fig of protein from heart extracts (.001 M KCN), with similar patterns observed for the 62 pigs among all breed groups. Further studies revealed that banding patterns were similar for cardiac and skeletal muscle and between pale, soft, exudative (PSE) and normal muscle at 5 min and 24 hr postmortem. The results suggest that previously reported variants in myoglobin in PSE skeletal muscle are not the result of alterations in primary structure.

¹ Scientific Journal Series Paper No. 10148, Minnesota Agricultural Experiment Station, St. Paul 55108. Presented at Western Section, Amer. Soc. Anim. Sci., Washington State University, Pullman, July 19, 1976.

² Department of Nutrition and Food Sciences, Utah State University, Logan 84322.

³ Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.

⁴ Current Address: Food Science Institute, Films-Packaging Division, Union Carbide, 6733 West 65th Street, Chicago, IL 60638.