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Effects of Certain Inorganic Elements and Urea onin Vitro Dry Matter Disappearance of Milo Stalks¹

New Mexico State University², Las Cruces 88003

Abstract

Ground milo stalks were digested with three levels of each calcium (10, 100 and 190 ppm), phosphorus (225, 350 and 475 ppm), magnesium (50, 100 and 150 ppm) and urea (.1, .3 and .5 g of nitrogen per liter) to determine effects on in vitro dry matter disappearance and to detect interactions among these nutrients. Treatment combinations were first screened to determine optimum levels of nutrients.

In experiment II, 10% of the ground milo stalks was replaced by 50 mg of glucose or starch with .1, .3, .5 or .7 g of nitrogen (as urea) per liter. The third experiment included iron (0 to 12 ppm), strontium (0 to 20 ppm), zinc (0 to 12 ppm), cadmium (0 to 5 ppm) and manganese (0 to 24 ppm).

Calcium at 190 ppm depressed in vitro dry matter disappearance. Phosphorus had no effect at the levels tested. Magnesium lowered digestibility linearly. Urea nitrogen depressed in vitro dry matter disappearance when levels exceeded .3 g per liter. Depressing effects of calcium, magnesium and urea-nitrogen appeared cumulative, as no interactions were observed in the experiment. Substitution of small quantities of fermentable carbohydrate for equal weight of milo stalks did not improve in vitro dry matter disappearance except at the highest level (.7 g/1) of urea-nitrogen.

Iron, strontium and zinc had no effect on in vitro dry matter disappearance of milo stalks. Manganese addition beyond 4 ppm tended to reduce in vitro dry matter disappearance, and a definite depression occurred with 24 ppm manganese. With 3 ppm cadmium, in vitro dry matter disappearance was higher than with 0, 1 or 2 ppm. Intermediate values were obtained with 4 and 5 ppm cadmium.

¹ Journal article 642, Agricultural Experiment Station, New Mexico State University, Las Cruces 88003.

² Departments of Animal and Range Science and Experimental Statistics.