J. Anim Sci.
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J. Anim Sci. 1978. 46:1063-1065.
© 1978 American Society of Animal Science
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Blockage of Sperm Transport Using Intraepididymal Calcium Chloride Injections in Rams1

T. A. Bowman, P. L. Senger, L. M. Koger2, C. T. Gaskins and J. K. Hillers

Washington State University3, Pullman 99164

Abstract

Ten mature rams were paired on the basis of pre-treatment semen production and assigned randomly to one of two treatments. Treatments were bilateral intraepididymal injections of 3 ml of 50% (w/v) CaCl2 in .9% NaCl (w/v) or 3 ml .9% NaCl. Semen was collected twice weekly for 5 weeks prior to treatment and for 7 weeks following treatment. Mounting times, ejaculate volume and sperm concentration were measured in all rams during the pre- and post-treatment periods. No differences between the groups were present for any characteristic during the pre-treatment period. However, reductions (P<.01) in sperm concentration and ejaculate volume were present by 12 days following CaCl2 injection. Mounting time was not affected by CaCl2 treatment. Mean values for the post-treatment period for the CaCl2 vs the saline treatments were, respectively: mounting time, 69 and 77 sec; ejaculate volume, .43 and .80 ml and sperm concentration, .15 and 3.96 x 109 sperm/milliliter. While the number of spermatozoa was never less than 105 sperm/ejaculate in the CaCl2-treated rams, all ejaculates were characterized by absence of motility and a predominance of fragmented spermatozoa.

Footnotes

1 Scientific Paper No. 4843. College of Agriculture Research Center, Washington State University, Pullman. Project No. 0237.

2 College of Veterinary Medicine.

3 Department of Animal Sciences.






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