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Guidelines for Hormone Radioimmunoassays¹

Hormone Subcommittee of A.S.A.S. Regulatory Agencies Committee

Abstract

Radioimmunoassays (RIA) are being used increasingly because of greater sensitivity and lower cost by comparison with biological or chemical assays. General principles of RIA have been discussed in each of the references listed at the end of this paper². The largest single problem in the use of RIA is adequate demonstration that the assay measures only the substance for which it is intended. The steps necessary to prove the validity of a RIA are straightforward. For example, if the RIA is for LH, it is necessary to demonstrate that other pituitary hormones do not interfere with the measurement of LH. If the assay is to be used to measure concentrations of LH in blood serum, then it is necessary to prove that other components of serum do not interfere with the determination of LH. The appropriate procedures for proof of the validity of RIA for protein (Midgley et al., 1969) and steroid (Niswender et al., 1975) hormones have been published.

¹ Reprints may be obtained from A.S.A.S. Business Manager, Claude Cruse, 311 Illinois Bldg., 113 N. Neil St., Champaign, IL 61820.