J. Anim Sci.
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J. Anim Sci. 1977. 45:832-836.
© 1977 American Society of Animal Science
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Isolation-Perfusion of Ovine Hind Limbs. III. Perfusate Lysine Levels1

J. A. Boling, J. C. Willard and W. R. Burris2

University of Kentucky,3, Lexington, 40506

Abstract

Fifteen wether lambs averaging 25.4 kg were utilized in a study to determine the influence of different perfusate lysine levels on amino acid metabolism in isolated-perfused ovine hind limbs. Lysine containing 50 µCi of 14C-L-lysine (UL) was added to the modified whole blood perfusate at levels equivalent to 0, 9 and 18 Mmole of added lysine per 100 ml of perfusate. Hind limb weights, perfusate pressure and flow rates did not change significantly (P<.05) during perfusion. Significant (P<.01) linear decreases in percent hematocrit were observed, but the changes were small in magnitude. A linear (P<.05) decrease was observed in perfusate pH in treatment 2, but overall values for treatments 1 to 3, respectively, were 7.36, 7.33 and 7.35 and were within the normal physiological range. Radioactivity of the plasma perfusate decreased curvilinearly (P<.01) with time for each treatment. Radioactivity of the deproteinized plasma perfusate decreased linearly (P<.01) with time of perfusion. Lysine uptake by muscle was increased (P<.10) when treatments 1 and 3 were compared, with treatment 2 having an intermediate uptake value. These data suggest that perfusate lysine concentration influences uptake by the muscle, but concentration changes do not adequately reflect uptake of amino acids in isolated perfused ovine hind limbs.

Footnotes

1 This Paper No. 76-5-105 is published with the approval of the Director of the Kentucky Agricultural Experiment Station.

2 Present address: Mississippi State University, South Miss. Branch Exp. Station, Poplarville.

3 Department of Animal Sciences.






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Copyright © 1977 by the American Society of Animal Science.