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Preparation of and Glucose Absorption from Intestinal Loops in Conscious Swine

Metabolism and Radiation Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Fargo, ND 58102

Abstract

Techniques are described for surgical preparation of isolated loops of small intestine in swine. Methods for training and post-surgical care of animals are outlined, and apparatus for perfusion of the intestinal loops are described. The viability of the loops was established by demonstration of active glucose transport. The rate of glucose absorption from isolated loops of duodenum and ileum was dependent upon the initial glucose concentration, and this relationship followed Michaelis-Menten kinetics. K_m and V_max coefficients are reported. Saturation and phlorizin inhibition of glucose transport were both demonstrated.