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Influence of Glycerol Levels, Diluent and Post-Thaw Temperature on Motility and Acrosomal Maintenance in Bovine Semen Frozen in Plastic Straws^{1 ,2,}

Washington State University^,4, Pullman 99163

Abstract

One ejaculate from each of 13 bulls was used to determine the influence of glycerol level on post-thaw susceptibility of spermatozoa in French straws to cold shock. Treatments were arranged factorially and included yolk-Tris and yolk-citrate diluents; 7, 9 and 11% glycerol; and exposure to 1, 20 or 37 C water baths for 3 min immediately post-thaw. Percentage of motile spermatozoa and percentage of intact acrosomes were quantitated after 0-, 4- and 8-hr incubation at 3 7 C to determine the effect of the post-thaw treatments on spermatozoa. Within each diluent and glycerol level, significant (P<.01) damage to cells resulted from the 1 C post-thaw treatment. Percentage of glycerol in the yolk-citrate diluent did not affect acrosomal maintenance in any of the post-thaw treatments. However, in the yolk-Tris diluent, 11% glycerol significantly (P<.01) reduced the percentage of intact acrosomes in all post-thaw treatments. Within the 7 and 9% glycerol levels Tris buffered semen had higher (P<.01) percentage of motile spermatozoa than the citrate buffered semen when both were exposed to the 20 C and 3 7 C post-thaw treatments.

¹ Scientific Paper No. 4679. College of Agriculture Research Center, Washington State University, Pullman. Project No. 4237

² Supported in part by Select Sires Inc., Plain City, OH 43064.

³ Select Sires Inc., Plain City, OH 43064.

⁴ Department of Animal Sciences.