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Ram Semen Prostaglandin Concentration and its Effect on Fertility>

Institute of Animal Science, 2232 Kostinbrod, Bulgaria

Abstract

The prostaglandins (PGs) have been isolated through an acid-ether extraction of ram seminal plasma and thin layer chromatography. The concentration of PGs was analyzed spectrophotometrically

A significant increase in the amount of PGE (E₁, E₂ and E₃) was found at the height of the breeding period due to the increased sexual activity of rams. No changes in the concentration of PGA (A₁ and A₂) and PGB (B₁ and B₂) were observed. There was no correlation between the concentration of prostaglandins and the volume of the ejaculates, number of or motility of spermatozoa.

The addition of PGE₂ and PGF₂ to the diluted ram semen in amounts comparable to the total amounts of PGs in one ejaculate increased the fertility of rams by more than 15%.

¹ Our thanks are due to Dr. John Pike of Upjohn Company, Kalamazoo for supplying us with PGE₂ and PGF₂, and to Dr. Krister Green from the Department of Medical Chem., Karolinska Institute, Stockholm for labelled PGE₂. The technical assistance of Mrs. Ts. Dacheva and Mrs. Z. Dodova is also acknowledged