HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rugg, C. D. Articles by Pickett, B. W. Search for Related Content PubMed PubMed Citation Articles by Rugg, C. D. Articles by Pickett, B. W.

Effect of Thawing Procedures on Fertility of Bovine Spermatozoa Frozen in .25-ml Straws¹

Colorado State University, Fort Collins 80523

Abstract

The influence of thawing procedures on fertility of semen in .2 5-ml Continental³ straws was investigated in a series of eight trials utilizing 1,634 cows. The following pregnancy rates were obtained: Trial 1, 38.6 vs 23.0% (P<.05) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 2, 29.7 vs 22.2% (P>.10) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 3, 82.8 vs 61.6% (P<.01) for semen thawed in 75 C water/7 sec vs "in the pocket"; Trial 4, 49.1 vs 45.9 (P>.10) for semen thawed in 75 C water/7 sec vs 3 C water/2 min; and Trial 5, 55.3 vs 56.0% (P>.10) for semen thawed in 75 C water/7 sec vs 35 C water/12 sec, respectively. Three of the eight trials were conducted to compare the fertility of semen used immediately after thawing or after incubation in iced water (3 C) for 10 minutes. All semen was thawed in water at 35 C/12 sec (Trial 6) or 75 C/7 sec (Trials 7 and 8). The pregnancy rates were as follows: Trial 6, 62.7 vs 47.6% (P<.05); Trial 7, 65.5 vs 59.7% (P>.10) and Trial 8, 70.8 vs 70.0% (P>.10), for semen used immediately or after 10 min of holding, respectively. It was concluded that thawing "in the cow" or "in the pocket" was unacceptable, and that semen in straws may be damaged by holding in iced water after thawing.

¹ Animal Reproduction Laboratory, Department of Physiology and Biophysics. Portions of these data are from theses submitted to the Colorado State University Graduate School in partial fulfillment of requirements for the Master of Science degree; and were presented, in part, at the XII Biennial Symposium on Animal Reproduction, 1975; the Sixth NAAB Technical Conference on Animal Reproduction and Artificial Insemination, 1976; and the Tenth Annual NAAB Conference on Artificial Insemination of Beef Cattle, 1976 and will appear in their respective proceedings. Thanks are extended to Achilles Cattle Co., Russell, Manitoba, Can. and Simmental Breeders Cardston Ltd., Cardston, Alberta, Can. for their donation of the semen used in these studies.

² Present address: Elgin Breeding Service, Box 468, Elgin, Texas 78621.