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Effects of in Vitro Incubation of Bovine Spermatozoa in Bovine Follicular Fluid¹

Oklahoma Agricultural Experiment Station, Stillwater 74074

Abstract

Addition of either 25% or 50% bovine follicular fluid (FF) to Krebs Ringer phosphate buffer, with .5% fructose added (KRPF), maintained higher percentages (P<.05) of motile sperm throughout 9-hr incubation at 37 C than did 100% KRPF or 100% FF. Accelerated sperm motility and head-to-head agglutination of sperm cells were consistently observed in all diluents that contained FF. The percentage of morphologically normal cells was not affected by any combination of FF and KRPF or by incubation. All concentrations of follicular fluid accelerated alteration of the acrosome with most of the alteration being apparent by 3 hr of incubation. Sperm treated with follicular fluid exhibited evident disintegration or detachment of the acrosome while cells treated with KRPF exhibited a slight thickening and roughening of the acrosome.

¹ Journal Article No. 3130 of the Agricultural Experiment Station, Oklahoma State University, Stillwater. Research conducted by the Department of Animal Sciences and Industry.

² Present address: University of Missouri, Columbia.

³ Department of Animal Sciences and Industry.