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In Vitro Studies on the Effect of Screening Processed Corn Distillers Solubles and Centrifuge Processed Corn Distillers Solubles on Cellulose Digestion and Microbial Protein Synthesis^{1 ,2,}

Purdue University, West Lafayette, IN 47907

Abstract

A series of in vitro fermentation trials were conducted to investigate the effect of screening processed distillers solubles (SDS) and centrifuge processed distillers solubles (CDS) on cellulose digestion, microbial protein synthesis and forage dry matter digestion. The possible difference between SDS and CDS was also surveyed by adding 1 ml each of valeric acid (10 mcg/ml), a mixture of biotin (20 mcg/ml) + PABA (25 mcg/ml) + vitamin B₆ (2 mg/ml) + vitamin B₁₂ (20 mcg/ml), or a mixture of L-proline (2 mg/ml) + L-leucine (2 mg/ml) + L-isoleucine (4 mg/ml) + L-valine (2 mg/ml) as a mixture to different treatments in various trials. The basal medium consisted of .5 g cellulose or 1 g orchardgrass, .5 ml urea solution (125 mg/ ml), 1 ml glucose (60 mg/ml), 30 ml McDougall's artificial saliva and 10 ml rumen fluid (250 g supernatant). The levels of SDS and CDS used included 0 (control), 25, 50, 75, 100 and 125 mg on dry matter basis.

Both SDS and CDS had significant effects (P<.01) on cellulose digestion at 50 mg and reached a maximum at 100 mg (SDS) and 125 mg (CDS). In a separate experiment, SDS (125 mg) increased cellulose digestion by 45% and CDS (125 mg) by 22%. When direct comparison at 125 mg level was made, the percent cellulose digestibilities were 50.7, 68.3 and 62.8, respectively, for control, SDS and CDS. The increase in microbial nitrogen after fermentation in g/ tube was 7.4, 13.3 and 9.6, respectively. The SDS had a significantly (P<.01) greater effect than did CDS on cellulose digestion and microbial protein synthesis. In these studies, the nutrient causing the difference between SDS and CDS or the rumen stimulatory factor of SDS or CDS was not valeric acid, a vitamin mixture or an amino acid mixture.

There was no difference between SDS and CDS in their effects on orchardgrass digestibility. In vitro digestibility percentages for the control, SDS and CDS, respectively, were: dry matter, 79.2, 76.4, 76.1 (P<01); organic matter, 79.1, 75.5, 75.3 (P<01); cell wall, 77.0, 75.7, 75.9; and cellulose, 79.7, 77.2, 77.6 (P<01).

¹ Journal Paper No. 6170, Purdue University Agricultural Experiment Station.

² The research reported herein was supported by a grant-in-aid from the Distillers Feed Research Council, Cincinnati, OH.

³ Present address: Taiwan Livestock Research Institute, Hsin-Hua, Tainan, Taiwan 712.

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