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Estimated Ruminal Production of Pseudovitamin B_{1 2}, Factor A and Factor B in Sheep¹

Cornell University^,3, Itbaca, NY 14853

Abstract

Digesta samples from experiments in which ruminal vitamin B_{1 2} production was quantitatively estimated were used for electrophoretic separation and estimation of ruminal production of pseudovitamin B_{1 2}, Factor A and Factor B. Mean estimates based on 61 samples representing a variety of diets were (µg/g digestible dry matter intake ± SE): pseudo-B_{1 2}, .52 ± .10; Factor A, 2.15 ± .19; Factor B, 1.24 ± .14; and B_{1 2}, 2.84 ± .17. Production of B_{1 2} and the analogues was differentially responsive to cobalt level in the diet with B _{1 2} being most responsive. Other dietary variables, including the general nature of the diet as well as chemical additives, were shown to influence production of the analogues. Alfalfa meal appeared to be especially effective in stimulating the synthesis of B_{1 2}, Factor A and Factor B.

¹ Supported in part by U.S. Public Health Service Grant AM14165.

² The authors gratefully acknowledge the assistance of Cynthia Walters, Mary Homan, Violet Topper and Lynn Polan.

³ Department of Animal Science.