J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1976. 43:809-815.
© 1976 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shea, B. F.
Right arrow Articles by Baker, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shea, B. F.
Right arrow Articles by Baker, R. D.

Maturation in Vitro and Subsequent Penetrability of Bovine Follicular Oocytes1

B. F. Shea2, J. P. A. Latour3, K. N. Bedirian4 and R. D. Baker

Macdonald Campus of McGill University5, Ste-Anne-de-Bellevue, Quebec HOA ICO

Abstract

To investigate optimum conditions for the resumption of meiosis in vitro, 450 bovine follicular oocytes were incubated for 0 to 36 hr in Ham's medium F-10 with 10% fetal calf serum. At recovery one oocyte was binucleate, 4% were degenerative and 8% had undergone germinal vesicle breakdown. After 28 hr in culture, 60% of the oocytes reached metaphase II and further incubation did not increase the proportion that matured. No significant difference was observed in the proportion of oocytes that matured in vitro from follicles flushed either 1 hr (78%) or 2 hr (72%) after the death of the donors. Oocytes matured in media with pH's ranging from 6.70 to 7.59. The highest proportion (69%) reached metaphase II in media at pH 7.00 to 7.29. Attempts to obtain sperm penetration of bovine oocytes incubated in vitro were successful in the pig oviduct (17%) but not in the sheep oviduct. In conclusion, under conditions which yielded a large proportion of metaphase II oocytes, these oocytes were able to be penetrated and form pronuclei.

Footnotes

2 This study was supported financially by Quebec Agriculture Research Council grant No. McA-72-491 and Medical Research Council grant No. 3711.

2 Present Address: University of Calgary, Division of Medical Biochemistry, Calgary, Alberta.

3 Department of Obstetrics and Gynaecology, Royal Victoria Hospital, Montreal.

4 Present Address: Via Pax Corporation Ltd., 7531 Pine Valley Drive, Woodbridge, Ontario.

5 Department of Animal Science.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the American Society of Animal Science.