J. Anim Sci.
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J. Anim Sci. 1976. 43:715-720.
© 1976 American Society of Animal Science
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Collection and Quantitation of Isotopically Labeled Acetic, Propionic and Butyric Acids

W. H. Hoover, P. H. Knowlton and E. E. Wildman1

University of Maine, Orono 04473

Abstract

A method was developed by which 14C labeled volatile fatty acids in aqueous solutions could be separated and collected directly into scintillation vials for radioassay. The instrument used was an Aerograph Model 705 gas chromatograph with a preparative size column packed with chromosorb 101 coated with 1% phosphoric acid. An effluent stream splitter in the system conducted part of the carrier gas to the flame ionization detector, and the remainder to a heated collector tip. The effluent stream from the collector tip was directed into the scintillation vial through a glass tube inserted through the vial cap. Loss of sample as an aerosol was prevented by wrapping .5 g glass wool moistened with the toluene-based scintillation fluid around the glass tube before insertion in the vial. As a further aid to entrapment, the vials were partially immersed in a –35 C methanol-dry ice bath during collection. After collection, the glass wool was stripped from the tube and left in the vial, the tube was rinsed and the vial filled with scintillation fluid. Tamping the glass wool was necessary to remove air bubbles. Means and standard deviations of the percent recoveries of 14 C labeled acetic, propionic and butyric acids in an aqueous mixture were 97.79 ± 3.36, 99.60 ± 1.65 and 100.63 ± 2.50, respectively.

Footnotes

1 Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg.






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Copyright © 1976 by the American Society of Animal Science.