J. Anim Sci.
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J. Anim Sci. 1976. 43:613-616.
© 1976 American Society of Animal Science
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Lactate Dehydrogenase Isoenzyme Patterns in Oocytes, Unfertilized Eggs and Embryos from Mice and Cattle

R. W. Wright, Jr.1, P. T. Cupps2, J. G. Watson3 and S. Chaykin3

University of California, Davis 95616

Abstract

Lactate dehydrogenase isoenzyme patterns in maternal tissues, oocytes, unfertilized eggs and embryos from mice and cattle were examined by polyacrylamide micro disc-gel electrophoresis. The results confirm that with respect to LDH content two types of oocytes, unfertilized eggs, and embryos contained only LDH-1 (ß subunits), while similar material from cattle exhibited LDH-1 and LDH-2 indicating the presence of both {alpha} and ß subunits. Maternal tissues such as heart, muscle and kidney, contained a greater number of lactate dehydrogenase isoenzymes than denuded oocytes, unfertilized eggs and embryos within species. Further, the intensity of the isoenzyme bands following staining increased toward LDH-4 in maternal tissues of cattle, while in denuded oocytes, unfertilized eggs and embryos of both the mouse and the cow the intensity increased toward LDH-1. These findings suggest that LDH activity found in oocytes, unfertilized eggs and embryos of mice and cattle is a result of de novo synthesis during oogenesis and/or the preimplantation period of development and not a result of contamination or uptake from maternal sources.

Footnotes

1 Present address: Department of Animal Sciences, Washington State University, Pullman 99163.

2 Department of Animal Science.

3 Department of Biochemistry and Biophysics.






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Copyright © 1976 by the American Society of Animal Science.