| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
University of Maine,2, Orono 04473
Abstract
An apparatus for the continuous culture of rumen microorganisms is described, which has a dual effluent removal system designed to simulate the differential flows for liquids and solids found in the rumen. In this apparatus a portion of the fermentation media is removed through a filter (primarily fluid), while mixed fluid and solid media flow out an overflow port. This permits a rapid buffer input rate for maintenance of pH levels, while allowing a longer residence time for digestion of solid particles. The maintenance of protozoa numbers in this system was found to be improved compared to reported numbers in single overflow systems operated at dilution rates greater than one. Feed rates, buffer flow rates, protozoa numbers and other fermentation parameters are reported. In addition, a feeding system for continuous culture studies is described which permits frequent, variable feeding of solid feeds.
1 The authors are indebted to L. L. Johnson for the calculations of solids turnover rates used in this manuscript, and to R. E. Goodnow for his assistance in developing the filter used in this procedure.
2 Department of Animal and Veterinary Sciences.
This article has been cited by other articles:
|
|
 |
|
  M. Lourenco, P. W. Cardozo, S. Calsamiglia, and V. Fievez Effects of saponins, quercetin, eugenol, and cinnamaldehyde on fatty acid biohydrogenation of forage polyunsaturated fatty acids in dual-flow continuous culture fermenters J Anim Sci, November 1, 2008; 86(11): 3045 - 3053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Calsamiglia, P. W. Cardozo, A. Ferret, and A. Bach Changes in rumen microbial fermentation are due to a combined effect of type of diet and pH J Anim Sci, March 1, 2008; 86(3): 702 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cerrato-Sanchez, S. Calsamiglia, and A. Ferret Effect of the magnitude of the decrease of rumen pH on rumen fermentation in a dual-flow continuous culture system J Anim Sci, February 1, 2008; 86(2): 378 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vazquez-Anon and T. Jenkins Effects of Feeding Oxidized Fat With or Without Dietary Antioxidants on Nutrient Digestibility, Microbial Nitrogen, and Fatty Acid Metabolism J Dairy Sci, September 1, 2007; 90(9): 4361 - 4367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cerrato-Sanchez, S. Calsamiglia, and A. Ferret Effects of Patterns of Suboptimal pH on Rumen Fermentation in a Dual-Flow Continuous Culture System J Dairy Sci, September 1, 2007; 90(9): 4368 - 4377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Fraser, A. V. Chaves, Y. Wang, T. A. McAllister, K. A. Beauchemin, and C. Benchaar Assessment of the Effects of Cinnamon Leaf Oil on Rumen Microbial Fermentation Using Two Continuous Culture Systems J Dairy Sci, May 1, 2007; 90(5): 2315 - 2328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cerrato-Sanchez, S. Calsamiglia, and A. Ferret Effects of Time at Suboptimal pH on Rumen Fermentation in a Dual-Flow Continuous Culture System J Dairy Sci, March 1, 2007; 90(3): 1486 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Castillejos, S. Calsamiglia, and A. Ferret Effect of essential oil active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. J Dairy Sci, July 1, 2006; 89(7): 2649 - 2658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rotger, A. Ferret, S. Calsamiglia, and X. Manteca Effects of nonstructural carbohydrates and protein sources on intake, apparent total tract digestibility, and ruminal metabolism in vivo and in vitro with high-concentrate beef cattle diets J Anim Sci, May 1, 2006; 84(5): 1188 - 1196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Busquet, S. Calsamiglia, A. Ferret, M. D. Carro, and C. Kamel Effect of Garlic Oil and Four of its Compounds on Rumen Microbial Fermentation J Dairy Sci, December 1, 2005; 88(12): 4393 - 4404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. D. M. Ribeiro, S. K. R. Karnati, and M. L. Eastridge Biohydrogenation of Fatty Acids and Digestibility of Fresh Alfalfa or Alfalfa Hay Plus Sucrose in Continuous Culture J Dairy Sci, November 1, 2005; 88(11): 4007 - 4017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Busquet, S. Calsamiglia, A. Ferret, P. W. Cardozo, and C. Kamel Effects of Cinnamaldehyde and Garlic Oil on Rumen Microbial Fermentation in a Dual Flow Continuous Culture J Dairy Sci, July 1, 2005; 88(7): 2508 - 2516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Cardozo, S. Calsamiglia, A. Ferret, and C. Kamel Effects of natural plant extracts on ruminal protein degradation and fermentation profiles in continuous culture J Anim Sci, November 1, 2004; 82(11): 3230 - 3236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Noftsger, N. R. St-Pierre, S. K. R. Karnati, and J. L. Firkins Effects of 2-Hydroxy-4-(methylthio) Butanoic Acid (HMB) on Microbial Growth in Continuous Culture J Dairy Sci, August 1, 2003; 86(8): 2629 - 2636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Thran, H. S. Hussein, D. Redelman, and G. C.J. Fernandez Influence of pH Treatments on Survival of Escherichia coli O157:H7 in Continuous Cultures of Rumen Contents Experimental Biology and Medicine, April 1, 2003; 228(4): 365 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miller-Webster, W. H. Hoover, M. Holt, and J. E. Nocek Influence of Yeast Culture on Ruminal Microbial Metabolism in Continuous Culture J Dairy Sci, August 1, 2002; 85(8): 2009 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wolin Fermentation in the rumen and human large intestine Science, September 25, 1981; 213(4515): 1463 - 1468. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
