J. Anim Sci.
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J. Anim Sci. 1976. 43:159-163.
© 1976 American Society of Animal Science
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Effect of selected Inhibitors and Methylene-Blue on a Possible Phosphogluconate Pathway in Washed Board Sperm1

W. L. Witters2 and C. W. Foley3

Ohio University, Athens, Ohio 45701

Abstract

The effects of 2mM iodoacetic acid, ImM sodium fluoride and methylene-blue dye were tested on washed boar sperm. Ten treatments (in duplicate) were used in four trials for each of the metabolic inhibitors iodoacetic acid and sodium fluoride on washed boar spermatozoa. The inhibitor or methylene-blue dye plus .5 µc of either glucose 1-14C or glucose 6-14C was paired with control flasks. Oxygen consumption was lower for both iodoacetate-treated (P<001) and sodium fluoride-treated (P<05) sperm. The pH was not significantly influenced by either inhibitor. Motility was lower (P<.01) for iodoacetic acid treatments but was not significantly different from controls for fluoride-treated samples. Iodoacetic acid-treated sperm produced (P<.01) greater 14CO2 from glucose l-14 C and significantly less 14CO2 from glucose 6-14 C. The phosphogluconate pathway was detected in iodoacetic acid-treated samples. Fluoride-treated sperm did not release more 14C02 from glucose 1-14C, but did (P<.01) decrease 14C02 from glucose 6-14C. No phosphogluconate pathway was distinguishable from data for fluoride treatments. The methylene-blue-treated samples had a higher release of 14 CO2 from glucose 1-14 C compared to glucose 6-14 C (P<.05). Data suggest that a phosphogluconate pathway was activated after treatment of sperm with methylene-blue dye.

Footnotes

1 This investigation was supported in part by NDEA Title IV Grant, 7654.

2 Associate Professor, Department of Zoology and Microbiology, Ohio University.

3 Professor of Veterinary Physiology, University of Missouri.






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