J. Anim Sci.
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J. Anim Sci. 1976. 42:951-954.
© 1976 American Society of Animal Science
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An Improved Method for Evaluating Acrosomes of Bovine Spermatozoa1

L. Johnson, W. E. Berndtson and B. W. Pickett

Colorado State University, Fort Collins 80523

Abstract

Studies were conducted in an effort to improve the convenience and objectivity by which the percentage of bovine spermatozoa with intact acrosomes (PIA) could be determined using differential-interference-contrast microscopy. The acrosomal integrity of spermatozoa from each of six bulls was evaluated in wet, seminal smears at 0, 2, 4 and 8 hr of post-thaw incubation at 37 C and compared to that of spermatozoa from the same samples fixed in buffered-glutaraldehyde solutions at each incubation interval, and evaluated after 0, 1, 8 or 29 days of room temperature storage. Spermatozoa were fixed in solutions of .2% glutaraldehyde in phosphate-buffered saline, with and without .15 M sodium cacodylate. When averaged over post-thaw incubation intervals and bulls, there was no difference (P>.25) in the PIA among unfixed samples and those preserved in either fixative solution and stored for up to 29 days. A higher percentage of fixed spermatozoa possessed intact acrosomes when the solution contained sodium cacodylate (P<.01).

In another experiment, raw semen from each of six bulls was incubated at 37 C for 0, 1, 2, 4 and 6 hours. At each incubation interval, aliquots of semem were evaluated in 2.9% sodium citrate or fixed and evaluated on days 1, 8 and 29 of storage. The PIA for unfixed samples was lower (P<.01) than for fixed samples (74 vs 89). Storage time had no effect (P>.10) on the PIA of fixed spermatozoa.

Footnotes

1 Animal Reproduction Laboratory, Department of Physiology & Biophysics. Supported in part by grants-in-aid from Cryenco and from Noba, Inc., through the National Association of Animal Breeders.






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