J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1976. 42:927-931.
© 1976 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pursel, V. G.
Right arrow Articles by Johnson, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pursel, V. G.
Right arrow Articles by Johnson, L. A.

Frozen Boar Spermatozoa: Methods of Thawing Pellets

V. G. Pursel and L. A. Johnson1

U. S. Department of Agriculture,2, 3,, Beltsville, Maryland 20705

Abstract

In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and motility of boar spermatozoa frozen by the pellet method were examined.

Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of normal apical ridge (NAR) acrosomes and motile spermatozoa at 3 minutes.

Thawing frozen pellets in solutions resulted in higher post-thaw percentages of NAR acrosomes than thawing in a Teflon-coated pan (P<.005); however, the opposite result was obtained for percentage of motile spermatozoa.

Thawing frozen pellets in a solution heated to 50 C resulted in higher percentages of NAR acrosomes and motile spermatozoa than thawing in a solution heated to 40 or 60 C.

Pellet volumes of .09, .18 or .27 ml had no effect on the percentage of NAR acrosomes after thawing; however, there was a significant positive linear relationship between pellet volume and motility (P<.005).

Footnotes

1 The authors wish to acknowledge the technical assistance of Leah L. Schulman.

2 Animal Physiology and Genetics Institute, Agricultural Research Service, Agricultural Research Center.

3 Mention of a trade name or proprietary product does not constitute a guarantee or warranty by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the American Society of Animal Science.