J. Anim Sci.
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J. Anim Sci. 1976. 42:121-130.
© 1976 American Society of Animal Science
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In vitro Steroidogenesis in Porcine Corpora Lutea1, 2,

J. R. Weiss3, H. J. Brinkley3 and E. P. Young4

University of Maryland, College Park

Abstract

Luteal tissue from nonpregnant sows was incubated with eight 14C-labeled precursors to determine the steroidogenic capabilities of this tissue. Eleven different 3H-steroids were used as internal indicators to permit the calculation of quantitative conversion data. The tissue was shown to be capable of transforming precursors to pregnenolone, progesterone, 17{alpha}-hydroxypregn-4-ene-3,20-dione, 20{alpha}- and 20ß-hydroxypregn-4-en-3-one, dehydroepiandrosterone, androstenedione, testosterone, estrone and estradiol. The {Delta} 5 pathway was investigated, and although transformations of a 17{alpha}-hydroxy pregnenolone to dehydroepiandrosterone and of dehydroepiandrosterone to androstenedione were demonstrated, the conversion of pregnenolone to 17{alpha}-hydroxypregnenolone could not be confirmed, suggesting that this pathway has limited, if any, significance in this tissue.

Footnotes

1 Reprint requests should be sent to H. J. Brinkley, Department of Zoology, University of Maryland, College Park 20742.

2 This investigation was supported by USPHS Research Grant HD 00739 from the National Institute of Child Health and Human Development and by Project C-34, Maryland Agric. Exp. Station. J. R. Weiss was supported by PHS Fellowship 4 F01 GM41338 and NASA Fellowship NsG(T)-3, Suppl. 3. Scientific Article No. A1699, Contribution No. 4460 of the Maryland Agr. Exp. Stat,, Department of Animal Science.

3 Department of Zoology, University of Maryland, College Park 20742.

4 Department of Animal Science, University of Maryland, College Park 20742.






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Copyright © 1976 by the American Society of Animal Science.