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Response of Ovine Pancreatic Amylase to Elevated Blood Glucose¹

University of Kentucky, Lexington 40506

Abstract

Response of pancreatic amylase to blood glucose was studied in wethers with re-entrant cannulae of the common bile duct. Combined biliary and pancreatic secretions were collected and assayed for amylase activity by a starch-iodine procedure before and after single intravenous injections of glucose or intravenous infusion of glucose for 3 hr out of each 12 for 3 or 7 days. Single injections containing 5 to 8 g of glucose or infusion of .5 ml per min of 8% glucose failed to significantly alter amylase secretion. Infusion of 16% glucose (.5 or 1 ml per min) sharply reduced amylase secretion (P<.01).

¹ The investigation reported in this paper (75-5-128) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the director.

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