HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Campion, D. R. Articles by Kuhlers, D. L. Search for Related Content PubMed PubMed Citation Articles by Campion, D. R. Articles by Kuhlers, D. L.

Mitochondrial Traits of Muscle from Stress-Susceptible Pigs¹

Iowa State University, Ames 50010

Abstract

The purpose of this investigation was to study the respiratory functions of skeletal muscle mitochondria isolated from 12 pigs of similar breeding that were equally divided as stress susceptible (SS) and stress resistant (SR), depending on their response to halothane (Fluothane®) anesthesia. Skeletal muscle samples were collected by biopsy 2 to 4 weeks before slaughter, immediately post-mortem and 3 hr post-mortem. As the muscle pH decreased from a mean value of 7.08 to 6.39, state 3 and state 4 respiration, respiratory control index and ADP/O ratio were reduced. No mitochondrial respiratory activity was observed below muscle pH 5.90. Statistical analysis was confined to biopsy and immediately post-mortem values because of lack of respiratory activity in all mitochondrial preparations from SS pigs at 3 hr post-mortem. For the remaining two sampling periods, no significant interactions were observed between time of sampling and type of pig for any of the respiratory traits measured. Type of pig did not show a significant relationship between state 3, state 4 or the ADP/O ratio when biopsy values were compared. Therefore, the mitochondrial characteristics studied do not appear to be involved in the etiology of the porcine stress syndrome. When muscle tissue was obtained immediately after death, the stress-susceptible pigs had significantly (P<.01) lower state 4 values, and the state 3 values were approaching significance (P<.07). The respiratory response probably was caused by a significantly (P<.01) lower muscle pH for the stress-susceptible pigs when evaluated immediately post-mortem.

¹ Journal Paper No. J-8102 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Projects 2008 and 1901.

² Present address: U.S. Meat Animal Research Center, A.R.S., U.S.D.A., Clay Center, Nebraska 68933.

³ Research supported by the Iowa Agriculture and Home Economics Experiment Station and by A.R.S., U.S.D.A. Cooperative Agreement No. 12-14-100-11185-44. Appreciation is expressed to Ayerst Laboratory for providing for Fluothane.