J. Anim Sci.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J. Anim Sci. 1975. 41:787-793.
© 1975 American Society of Animal Science
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dildey, D. D.
Right arrow Articles by Judge, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dildey, D. D.
Right arrow Articles by Judge, M. D.

Investigation of Trypan Blue Dye for Determination of Circulatory Capacity of Skeletal Muscle1

D. D. Dildey, E. D. Aberle, J. C. Forrest, C. W. Alliston and M. D. Judge

Purdue University, West Lafayette, Indiana 47907

Abstract

The use of trypan blue dye to determine circulatory capacity of skeletal muscle was evaluated in rabbits and pigs. The technique was adequate for this purpose if certain precautions were observed. Recovery of dye from either blood plasma or muscle presented no procedural difficulties, but quantitative determination of dye in whole blood appeared to be confounded by the presence of hemoglobin.

Injection of the dye at dosages of greater than .2 g/kg of body weight gave a linear increase in plasma dye concentration with increasing dose level, thus a dose rate of .3 g/kg body weight was used in subsequent experiments. Higher dose levels resulted in higher muscle concentrations of dye, which indicated the necessity to inject a standard dose in order to make comparisons among animals. Residual dye in the blood may interfere with accurate estimation of circulatory capacity if sampling is done prior to 8 hr postinjection and 24 to 30 hr postinjection in rabbits and pigs, respectively. Since dye was slowly cleared from muscle postinjection, the time of sampling should be closely standardized among animals. Muscles known to contain higher proportions of red fibers and greater numbers of capillaries bound greater quantities of dye than muscles with fewer red fibers or lower numbers of capillaries.

Footnotes

1 Department of Animal Science. Journal Paper No. 5758. Agriculture Experiment Station.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Society of Animal Science.