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Prostaglandins F in Uterine Tissue and Venous Plasma of Ewes with Intrauterine Devices¹

West Virginia University, Morgantown 26506

Abstract

Levels of prostaglandins F (PGF) in uterine tissues and in uterine and peripheral plasma from ewes with and without IUD's were examined. Mature ewes were laparotomized on day 2 of the estrous cycle. Fifteen ewes with a corpus luteum on only one ovary were assigned at random to either a treated group (10 ewes) which received an intrauterine device (IUD) in each uterine horn or to a control group (five ewes). Twelve ewes had a corpus luteum on each ovary and received at random an IUD in one uterine horn (eight ewes) or a sham operation (four ewes). Blood was collected from a major vein of each uterine horn on day 5. Uteri were removed and the uterine horns were separated and dissected into caruncular and intercaruncular endometrium and myometrium. Prostaglandins F were extracted with ethyl acetate, separated by column chromatography on silicic acid and quantified by radioimmunoassay.

An IUD in one or in both uterine horns resulted in higher concentrations (ng/ml) of PGF in uterine venous plasma from both horns (6.4 ± 2.2 to 9.3 ± 2.7) than in controls (.5 ± .1 to .6 ± .2). PGF (ng/g) in uterine tissue from ewes which had an IUD in each uterine horn was considerably higher in caruncular (382 ± 42), intercaruncular (641 ± 95) and myometrial tissue (82 ± 9) than in controls (199 ± 49, 240 ± 72 and 50 ± 11 in caruncular and intercaruncular endometrium and myometrium, respectively). In bilaterally-ovulating ewes with an IUD in one uterine horn, caruncular endometrium from those horns consistently contained more PGF (293 ± 23 ng/g) than caruncular endometrium from uterine horns not containing an IUD (149 ± 21) or from control ewes (130 ± 24). The concentration of PGF in jugular plasma from IUD-bearing ewes (.4 ± .1) was not different from that measured in plasma from control ewes (.4± .1).

¹ Published with the approval of the Director of the Agriculture Experiment Station as Scientific Paper No. 1337. Division of Animal and Veterinary Science. Supported by NIH contract 69-2215. We thank The Upjohn Company and Dr. John E. Pike for generous supplies of prostaglandins E₁, E₂, F₁, F₂, 15-keto F₂ and 13, 14-dihydro-15-keto F₂. The authors gratefully acknowledge Phyllis Jenkins and Maryann Kline for technical assistance and Dr. W. V. Thayne and Dr. E. J. Harner, Jr. for assistance in statistical analysis.

² West Virginia University Foundation Graduate Research Fellow.

³ Present address: Department of Animal Science, Oregon State University, Corvallis 97331.

⁴ Present address: Center for Endocrinology, Metabolism and Nutrition, Northwestern University, Chicago, Illinois 60611.

⁵ Department of Obstetrics and Gynecology.