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Development of Rabbit Embryos after Storage at 10 C¹

Cornell University, Ithaca, New York 14853³

Abstract

Two-cell rabbit embryos were stored at 10 C for 24 hr in BSM II, a chemically defined medium, or BSM II with 4% gelatin, 4% Promine-D, or 3% egg yolk lipoprotein added for cryoprotection. Following storage and subsequent transfer to recipients, embryos were allowed to develop to term. The addition of 4% gelatin or 4% Promine-D to the storage medium was beneficial and resulted in a rate of development similar to embryos which were not stored at 10 C, but were transferred immediately after collection. Increasing the period embryos were stored in BSM II plus 4% Promine-D to 48, 72 and 96 hr resulted in a progressive decrease in viability. Regardless of the storage medium or the storage period, losses due to low temperature storage were shown to occur prior to day 12 of gestation. Postnatal development of treated embryos, as determined by 3-week weight gain, was equivalent to control embryos.

¹ This research was supported in part by NIH Research Grant HD 03471. The authors thank Marilee Allen, Michael Simkin and Richard Cole for their excellent assistance.

² Present address: Department of Animal Science, University of California, Davis 95616.

³ Department of Animal Science and Division of Biological Science.