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Alteration of Phospholipids in Porcine Spermatozoa during In Vivo Uterus and Oviduct Incubation^{1, 2,}

Purdue University, West Lafayette, Indiana 47907

Abstract

Pig spermatozoa were incubated in ligated segments of the uterus and oviduct of 12 gilts to investigate the influence of the female reproductive tract on sperm phospholipids. Sperm-rich semen was incubated in vivo for 30 to 120 min in prepuberal gilts with induced estrus. Control samples were incubated in vitro for up to 40 minutes. No significant alterations were observed in the in vitro incubations. Uterine incubation for 90 min resulted in a significant (P < .05) decline in phosphatidylcholine (PC), but incubation for 30 min more appeared to restore PC to the levels of the unincubated controls. Uterine incubation also resulted in apparent synthesis of phosphatidylinositol (PI), a compound that was not present in freshly-ejaculated sperm or in sperm incubated in vitro. During incubation in the oviduct, sperm PI appeared more rapidly than during uterine incubation, and cardiolipin levels decreased significantly (P <.05).

¹ Journal Paper No. 5327, Purdue University Agricultural Experiment Station, Department of Animal Sciences.

² Research was supported by a Merck Grant for Faculty Development and NIH Grant HD07013.