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Porcine Spermatozoan Respiration and Motility in the Presence of Bovine Follicular Fluid^{1, 2,}

University of Missouri⁴, Columbia 65201

Abstract

Respiration and motility of washed porcine spermatozoa in the presence of bovine follicular fluid were studied. A Gilson Differential Respirometer was used to measure respiration rates (ZO₂ values). Final motility scores were visually estimated using a microscope.

Whole bovine follicular fluid (WBFF) as 5, 10 or 20% of the suspension medium had a detrimental effect (P< .05) on respiration rates and motility as well as caused head-to-head agglutination relative to spermatozoa suspended entirely in Ca⁺⁺ -free Krebs-Ringer phosphate buffer (KRPG) containing 1 mg glucose per milliliter. Dialyzed (DBFF) or heated (30 min at 60 C) bovine follicular fluid (HBFF) was less toxic to spermatozoa than WBFF. Spermatozoa suspended in 20% HBFF exhibited significantly higher (P< .05) respiration rates than KRPG suspended controls.

¹ Contribution from the Missouri Agricultural Experiment Station. Journal Series No. 6351. Approved by the Director.

² This investigation was supported in part by a PHS Training Grant HD-00170 from the National Institutes of Health, Public Health Service. We acknowledge assistance with statistical analysis by Marvin Frick and with collection of semen and follicular fluid by R. K. Christenson, C. E. Pope, V. Zimmerman-Pope and J. Summers.

³ Present address: Department of Physiology, University of Kansas Medical Center, Rainbow Boulevard at 39th Street, Kansas City 66103.

⁴ Departments of Agricultural Chemistry and Animal Husbandry.