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Biuretolytic Activity of Rumen Microorganisms as Influenced by Dietary Levels of Biuret, Molasses and Dehydrated Alfalfa^{1, 2,}

Oklahoma Agricultural Experiment Station, Stillwater 74074

Abstract

Three experiments were conducted to study the effects of varying dietary levels of biuret, dehydrated alfalfa and molasses upon biuret adaptation by rumen microorganisms. When biuret was fed as 20, 50 and 80% of the total dietary nitrogen in a high cottonseed hull ration, rate and extent of biuretolytic activity development was greatest at high biuret levels. When biuret supplied 50 and 80% of the total dietary nitrogen, BA was significantly (P< .05) greater at day 14 than BA at 20% biuret and this difference was maintained for the remainder of the 35 day trial.

In experiment two similar rations containing 50% of the total dietary nitrogen as biuret, were supplemented with 0, 2 and 8% dehydrated alfalfa both with and without 5% molasses. Biuret adaptation was rapid on all diets, generally being complete by day 3 of the trial. No significant (P> .05) treatment effects were observed. When 5% molasses was present, a slight advantage favoring the 0 and 2% alfalfa levels was evident.

¹ Journal Article 2827 of the Agricultural Experiment Station, Oklahoma State University, Stillwater.

² Supported in part by the Lone Star Gas Company, Dallas, Texas.

³ Department of Animal Sciences and Industry, Oklahoma State University, Stillwater 74074.

⁴ Present address: Dept. of Animal Science, University of Tennessee, Knoxville 37901.

⁵ Present address: Dept of Physiology and Biochemistry, New York State Veterinary College, Cornell University, Ithaca 14850.