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Livability and Glycolysis of Bovine Spermatozoa in Yolk-citrate, Incubated Eggs or Chick-Embryo Diluters

Cornell University

Abstract

Two different experiments were conducted to determine the value of a chick-embryo diluter as compared to the yolk-citrate. The chick-embryo diluent (C.E. I) made from whole-fertile-eggs incubated 9 to 11 days was only slightly superior in maintaining livability of the spermatozoa during low-temperature storage to the yolk-citrate. The difference was observed largely with the semen samples of poorest original quality, which ordinarily would not be used in commercial artificial insemination. In a limited experiment with semen of better average quality no difference was noted in spermatozoa livability during storage between yolk-citrate, chick-embryo made from whole-fertile-incubated eggs, and a diluter made from the embryos only.

The semen samples in the chick-embryo I diluent produced slightly more lactic acid during storage than did the same semen samples in the yolk-citrate. Also, a larger proportion of the glucose lost on storage was recovered as lactic acid in the chick-embryo diluents. This fact suggests that the chick-embryo material aided in the promotion of glycolysis by the spermattozoa.