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Serum and Pituitary LH Concentrations in Ewes Infused with LH-RH/FSH-RH^{1, 2, 3,}

Washington State University⁴, Pullman 99163

Abstract

Twenty anestrous ewes (mean body weight 68 kg) were assigned at random in a 2 x 2 factorial experiment. The ewes were continuously infused with synthetic LH-RH/FSH-RH or acidified saline for a period of 24 hours. Half of the ewes in each group were pretreated with a synthetic progestagen (flurogestone acetate) for 14 days prior to infusion. Serum and pituitary LH concentrations, serum estrogen concentration, stalk median eminence (SME) LH-releasing activity, and ovarian characteristics were studied.

Infusion with LH-RH/FSH-RH at the rate of 2.3 µg/hr. induced a significant increase in serum LH concentrations, which peaked (23 to 30 ng/ml) at 3 hr. and decreased to pretreatment LH concentration (0.7 ng/ml) by 16 hours. There were no differences in peak serum LH concentrations or duration of LH response due to pretreatment with the synthetic progestagen. Infusion with LH-RH/FSH-RH lowered pituitary LH content and concentration but had no effect on serum estrogen levels, pituitary dry weight, stalk median eminence LH-releasing activity, ovarian weight, or ovarian follicular diameter.

¹ Scientific Paper 4177, College of Agriculture Research Center, Washington State University. Project 0137.

² The authors would like to thank Dr. Y. Baba of Sankyo Co. Ltd., Tokyo, Japan for providing us with pure synthetic LH-RH/FSH-RH; Dr. L. Reichert for ovine LH used for iodination; Dr. W. White for antiovine LH serum used for quantitating ovine LH levels; Dr. G. D. Niswender for antiovine LH serum used for quantitating murine LH levels; Dr. T. M. Nett and Dr. V. L. Estergreen for antiestrogen serum used for quantitating total estrogens and the Endocrine Study Section NIH for LH standards.

³ This study was supported in part by USPHS Grant from the National Institute of Child Health and Human Development AM 06192.

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