HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pace, M. M. Articles by Graham, E. F. Search for Related Content PubMed PubMed Citation Articles by Pace, M. M. Articles by Graham, E. F.

Components in Egg Yolk which Protect Bovine Spermatozoa during Freezing¹

University of Minnesota, St. Paul 55101

Abstract

Bull spermatozoa were pellet frozen on dry ice in a TES buffer containing combinations of egg yolk and glycerol to determine which component of an extender afforded cryoprotection to the spermatozoa. Neither buffer nor buffer plus glycerol protected sperm cell motility in the absence of egg yolk, while sperm cells frozen in egg yolk buffer in the absence of glycerol had 24% postthaw motility (P< .05). Egg yolk was found to be the main cryoprotective agent, but there was a synergistic effect between glycerol and egg yolk in providing the greatest postthaw survival (40%) of sperm cells (P< .05).

Purification of egg yolk to determine which fractions were affording protection was conducted using ultracentrifugation, Biogel filtration and Sephadex filtration. Purification procedures and agar gel electrophoresis indicated that a large lipoprotein complex could be isolated free of contaminating migrating proteins. The lipoprotein complex was the low density fraction (LDF) of egg yolk. In the absence of glycerol this complex protected motility of sperm cells during the freezing process.

¹ Scientific Journal Series, Paper No. 7941, Minnesota Agricultural Experiment Station, St. Paul.

² Present address: American Breeders Service, DeForest, Wi. 53532.

This article has been cited by other articles:

				A. Bergeron, Y. Brindle, P. Blondin, and P. Manjunath Milk Caseins Decrease the Binding of the Major Bovine Seminal Plasma Proteins to Sperm and Prevent Lipid Loss from the Sperm Membrane During Sperm Storage Biol Reprod, July 1, 2007; 77(1): 120 - 126. [Abstract] [Full Text] [PDF]

				A. Bergeron, M.-H. Crete, Y. Brindle, and P. Manjunath Low-Density Lipoprotein Fraction from Hen's Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm Membrane Biol Reprod, March 1, 2004; 70(3): 708 - 717. [Abstract] [Full Text] [PDF]