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Isolation-Perfusion of Ovine Hind Limbs I. Development of Procedures¹

University of Kentucky⁴, Lexington 40506

Abstract

A technique utilizing an artificial heart-lung system for isolation-perfusion of ovine hind limbs was described. Twelve lambs were used in a preliminary study to develop the surgical and perfusion technique. Rising perfusion pressure, falling venous output and increasing tissue edema caused a termination of most of the preliminary studies within 15 to 30 min. after isolation of the hind limbs. Varying levels of phenoxybenzamine was ineffective in relieving these conditions. However, addition of 150 ml of a 10% dextran (mw 40,000) Krebs-Ringer-bicarbonate solution to 2.15 liters of the whole blood perfusate relieved the symptoms of organ distress. The hind limbs from seven additional wethers were isolated and perfused for a 2-hr, period. Data concerning the perfusion technique and tissue viability are reported.

¹ The investigation reported in this paper (73-5-167) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with approval of the Director.

² Present address: Clemson University, Clemson, South Carolina 29631.

³ Appreciation is expressed to Armour Packing Co., Lexington, Kentucky for supplying whole ovine blood and to Mr. T. L. Redd for technical assistance.

⁴ Department of Animal Sciences.